LOCUS       pQE-3.        3416 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial expression vector with a T5 promoter. For other reading 
            frames, use pQE-4 or pQE-5.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-3.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    121..146
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    146..156
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      162..256
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             300..956
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1024..1110
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1266..1406
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2354..3211)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3212..3316)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatccgtcg acctgcagcc aagcttaatt agctgagctt ggactcctgt tgatagatcc
      181 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      241 ttttattggt gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa
      301 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      361 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      421 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      481 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
      541 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      601 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      661 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      721 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      781 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      841 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
      901 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
      961 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctcctgag taggacaaat ccgccgctct agagctgcct cgcgcgtttc
     1141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1261 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1321 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1381 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1441 gctcggtctg tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2401 gttcatccat agctgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3361 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//
