LOCUS       pQE-10.        3442 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing N-terminally 6xHis-tagged proteins. 
            For other reading frames, use pQE-9 or pQE-11.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-10.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    147..172
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    172..182
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      188..282
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             326..982
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1050..1136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1292..1432
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1618..2206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2380..3237)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3238..3342)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatctcacc atcaccatca ccatacggat ccgtcgacct gcagccaagc ttaattagct
      181 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      241 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag
      301 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      361 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      421 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      481 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      541 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      601 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      661 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      721 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
      781 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
      841 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
      901 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
      961 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     1021 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
     1081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1141 cgctctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1261 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     1321 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     1381 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     1441 cgcttcctcg ctcactgact cgctgcgctc ggtctgtcgg ctgcggcgag cggtatcagc
     1501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1801 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2041 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2401 acctatctca gcgatctgtc tatttcgttc atccatagct gcctgactcc ccgtcgtgta
     2461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3361 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3421 cacgaggccc tttcgtcttc ac
//
