LOCUS       40924_29781        7632 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 7632)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..1320
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     intron          1562..1627
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1757..1777
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2202..2336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2349..2678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2878..3333
                     /gene="gpt"
                     /label=gpt
                     /note="Xanthine-guanine phosphoribosyltransferase from 
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC). 
                     Accession#: B7N8G9"
     intron          3813..3878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4008..4028
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4453..4587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5440..5544
                     /label=AmpR promoter
     CDS             5545..6402
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6576..7164
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7350..7490)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 aagcttgggc agaaatggtt gaactcccga gagtgtccta cacctagggg agaagcagcc
       61 aaggggttgt ttcccaccaa ggacgacccg tctgcgcaca aacggatgag cccatcagac
      121 aaagacatat tcattctctg ctgcaaactt ggcatagctc tgctttgcct ggggctattg
      181 ggggaagttg cggttcgtgc tcgcagggct ctcacccttg actcttttaa tagctcttct
      241 gtgcaagatt acaatctaaa caattcggag aactcgacct tcctcctgag gcaaggacca
      301 cagccaactt cctcttacaa gccgcatcga ttttgtcctt cagaaataga aataagaatg
      361 cttgctaaaa attatatttt taccaataag accaatccaa taggtagatt attagttact
      421 atgttaagaa atgaatcatt atcttttagt actattttta ctcaaattca gaagttagaa
      481 atgggaatag aaaatagaaa gagacgctca acctcaattg aagaacaggt gcaaggacta
      541 ttgaccacag gcctagaagt aaaaaaggga aaaaagagtg tttttgtcaa aataggagac
      601 aggtggtggc aaccagggac ttatagggga ccttacatct acagaccaac agatgccccc
      661 ttaccatata caggaagata tgacttaaat tgggataggt gggttacagt caatggctat
      721 aaagtgttat atagatccct cccttttcgt gaaagactcg ccagagctag acctccttgg
      781 tgtatgttgt ctcaagaaga aaaagacgac atgaaacaac aggtacatga ttatatttat
      841 ctaggaacag gaatgcactt ttggggaaag attttccata ccaaggaggg gacagtggct
      901 ggactaatag aacattattc tgcaaaaact catggcatga gttattatga atagccttta
      961 ttggcccaac cttgcggttc ccagggctta agtaagtttt tggttacaaa ctgttcttaa
     1021 aacgaggatg tgagacaagt ggtttcctga cttggtttgg tatcaaaggt tctgatctga
     1081 gctctgagtg ttctattttc ctatgttctt ttggaattta tccaaatctt atgtaaatgc
     1141 ttatgtaaac caagatataa aagagtgctg attttttgag taaacttgca acagtcctaa
     1201 cattcacctc ttgtgtgttt gtgtctgttc gccatcccgt ctccgctcgt cacttatcct
     1261 tcactttcca gagggtcccc ccgcagaccc cggcgaccct caggtcggcc gactgcggca
     1321 gctggcgccc gaacagggac cctcggataa gtgacccttg tctctatttc tactatttgg
     1381 tgtttgtctt gtattgtctc tttcttgtct ggctatcatc acaagagcgg aacggactca
     1441 ccatagggac caagctagcc cggggtcgac cggtcgacct cgagggatct ttgtgaagga
     1501 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     1561 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     1621 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     1681 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     1741 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     1801 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     1861 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     1921 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     1981 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     2041 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     2101 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2161 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggc
     2341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     2701 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     2761 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     2821 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     2881 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     2941 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     3001 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     3061 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     3121 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     3181 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     3241 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     3301 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     3361 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     3421 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     3481 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     3541 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     3601 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     3661 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     3721 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     3781 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     3841 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     3901 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     3961 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     4021 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     4081 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     4141 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     4201 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     4261 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     4321 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     4381 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4441 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4501 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4561 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
     4621 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     4681 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     4741 tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     4801 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
     4861 cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
     4921 aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
     4981 atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
     5041 agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
     5101 tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
     5161 aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
     5221 ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
     5281 ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattct
     5341 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5761 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6061 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     6121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6721 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7321 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7381 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7441 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     7501 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7561 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7621 gcgcgaggca gc
//
