LOCUS       pLenti6_TR.        8354 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector for high-level constitutive expression of the Tet 
            repressor.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti6 TR.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2552..3124
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        3179..3197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3208..3828
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             3870..3899
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     promoter        3947..4276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4324..4371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4390..4785
                     /label=BSD
                     /note="blasticidin S deaminase"
     LTR             4875..5108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5180..5314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5341..5476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5497..5515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5525..5541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5683..6138
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6164..6268
                     /label=AmpR promoter
     CDS             6269..7126
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7300..7888
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8176..8197
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8212..8242
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8266
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8311..8329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatagctt ggtacccggg gatcctctag
     2461 ggcctctgag ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa
     2521 aaagctccgg atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     2581 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     2641 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     2701 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     2761 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     2821 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     2881 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     2941 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     3001 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     3061 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     3121 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta
     3181 atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac
     3241 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     3301 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     3361 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     3421 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     3481 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     3541 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     3601 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     3661 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     3721 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     3781 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga
     3841 tcccgggaat tctagagggc ccgcggttcg aacaaaaact catctcagaa gaggatctga
     3901 atatgcatac cggttagtaa tgagtttgga attaattctg tggaatgtgt gtcagttagg
     3961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     4321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     4381 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     4441 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     4501 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     4561 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     4621 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     4681 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     4741 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acaattcgag
     4801 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4861 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4921 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4981 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5041 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5101 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5161 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5281 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5461 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5521 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5581 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5641 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5701 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5761 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5821 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5881 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5941 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6001 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6061 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6121 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6241 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6481 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6721 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6781 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6841 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6901 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6961 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7021 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7081 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7141 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7201 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7441 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7921 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7981 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8041 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8101 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8161 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8221 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8281 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8341 ggagctgcaa gctt
//
