LOCUS       pETcoco-2.       12417 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector with an ampicillin resistance marker that allows 
            single-copy replication, on-demand amplification, and tightly 
            regulated expression.
ACCESSION   .
VERSION     .
KEYWORDS    pETcoco-2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 12417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The copy number can be increased to 20-50 copies per cell by adding 
            arabinose to the growth medium.
FEATURES             Location/Qualifiers
     source          1..12417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             72..74
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             75..92
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             102..146
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             162..176
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             249..281
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             326..343
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      410..457
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(834..1709)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1736..2020
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2080..3225
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(3449..4306)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4307..4398)
                     /label=AmpR promoter
     rep_origin      complement(4965..5677)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5756..5975
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6066..6818
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    6824..7074
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7400..8572
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             8575..9543
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    9619..10092
                     /gene="sopC"
                     /label=sopC
                     /note="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(10352..10750)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(10768..10801)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(10894..10915)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(10931..12010)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(12011..12088)
                     /label=lacI promoter
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataag catgcatcac catcaccatc acggtgctag caaagaaacc gctgctgcga
      121 aatttgaacg ccagcacatg gactcgccac cgccaactgg tgatgacgac gacaagagcc
      181 cgggcgatcg catcgatgac gtccaggcgc gccacgtgcg tacggtttcg aagcttgcgg
      241 ccgcaagcca gccagaactc gctccggaag atcccgagga tctttaatta acctcaggat
      301 ttaaataacc taggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      721 tcacggccgc cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg
      781 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac
      841 ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct cgggctggcc
      901 ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga
      961 ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg gctgatacgt
     1021 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
     1081 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
     1141 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg cggatggagc
     1201 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
     1261 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
     1321 aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa tattgacggc
     1381 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
     1441 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
     1501 tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
     1561 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
     1621 ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc
     1681 agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa
     1741 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
     1801 ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
     1861 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
     1921 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     1981 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag
     2041 tgaaaggagg aattcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg
     2101 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc
     2161 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc
     2221 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc
     2281 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg
     2341 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc
     2401 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag
     2461 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg
     2521 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc
     2581 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc
     2641 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggctgca ggtgttggag
     2701 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag
     2761 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc
     2821 ctacaggcga cggcgatgga cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg
     2881 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg
     2941 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag
     3001 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg
     3061 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc
     3121 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg
     3181 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg
     3241 tcagttccgg ctgggggttc agcagccacc gggccgtcga ccaattctca tgtttgacag
     3301 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
     3361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     3421 cagttgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4381 tttagaaaaa taaacaaata ggggtttacc gctgaaagtt ctgcaaagcc tgatgggaca
     4441 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg
     4501 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc
     4561 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt
     4621 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa
     4681 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata
     4741 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt
     4801 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa
     4861 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta
     4921 gtgctcgccg cagtcgagcg acagggcgaa gccctcgaga tcccctgcca tttttggggt
     4981 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggc
     5041 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
     5101 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
     5161 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
     5221 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
     5281 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
     5341 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
     5401 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
     5461 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
     5521 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg
     5581 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag
     5641 cggcgagggc aaccagcccg gtgagcgtcg gaaaggggaa tttcgagtga gcgaggaagc
     5701 accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa cttcccttgg
     5761 ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt ttttagatct
     5821 tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag gtgttgtgac
     5881 aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg
     5941 cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa gttatccctg
     6001 cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact tcacatggat
     6061 ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg
     6121 tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa aaacgtatgc
     6181 tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa catgacggta
     6241 tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct gcggaagcca
     6301 gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc
     6361 ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt atcaaacgtg
     6421 cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc attcccttct
     6481 ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca
     6541 atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg gatggctcag
     6601 gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct caaagttacc
     6661 agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag atcaacagca
     6721 gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg actcatatcg
     6781 tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt tatctgtcac
     6841 agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt gtcacagttt
     6901 tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa tttttaagga
     6961 agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt cgtcatgtga
     7021 cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca gtttattact
     7081 ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt ggatatttct
     7141 tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc ctcgccagtt
     7201 cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag tgactgaggt
     7261 atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact ttgcggtttt
     7321 ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta ataaaaaaac
     7381 gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta accagtgcat
     7441 aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg atgacagccc
     7501 ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt tagttggggt
     7561 ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc cggatatgga
     7621 aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta atcatatgcg
     7681 tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg tgatcggggt
     7741 tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg ctcaggatct
     7801 ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg gaacagcctc
     7861 aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc tcctgccttt
     7921 ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct ggccggggct
     7981 tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa tgggcaaatt
     8041 tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca ttgaaactgt
     8101 tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta tcggcacgat
     8161 taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt tgtttgacta
     8221 cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg ttgatcttaa
     8281 agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta atggctctca
     8341 gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc taaaaaatgt
     8401 tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg tttttgaaca
     8461 ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta tttgggaacc
     8521 tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta gataatgaag
     8581 cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga tacttcgtta
     8641 tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt aatggctcgc
     8701 ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct tgaagtgctc
     8761 cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg tgaccaggag
     8821 ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac tggtcaacag
     8881 acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg gagtcgccgt
     8941 cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga gctggatgat
     9001 gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag tgcttatgaa
     9061 cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat ttctgcgctg
     9121 gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac cgccaaattg
     9181 cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg gtcaggtgat
     9241 gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc atctaacctt
     9301 catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac tcttttaact
     9361 tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca tcagtttgct
     9421 cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga caggtctcgt
     9481 gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga aaagccagca
     9541 ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt cctttgttac
     9601 aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc acttgtatcg
     9661 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt
     9721 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
     9781 ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg
     9841 attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     9901 acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc cactcgtatc
     9961 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
    10021 tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga ccacggtccc
    10081 acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg cctgtcaagg
    10141 gcaagtattg acatgtcgtc gtaacctgta gaacggagta acctcggtgt gcggttgtat
    10201 gcctgctgtg gattgctgct gtgtcctgct tatccacaac attttgcgca cggttatgtg
    10261 gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa
    10321 gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg ttgccccgta ttcagtgtcg
    10381 ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa ttaatacgat
    10441 acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg ttgtgatatg
    10501 tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg ttacggggcg
    10561 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt
    10621 cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg aaacgacagg
    10681 tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg
    10741 aacaatggaa gtccgagctc atcgctaata acttcgtata gcatacatta tacgaagtta
    10801 tattcgatgc ggcctagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
    10861 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
    10921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
    11041 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
    11101 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
    11161 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
    11221 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
    11281 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
    11341 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
    11401 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
    11461 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
    11521 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
    11581 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
    11641 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
    11701 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
    11761 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
    11821 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
    11881 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
    11941 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
    12001 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
    12061 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
    12121 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
    12181 ggtgcatgta aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
    12241 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
    12301 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
    12361 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactata
//
