LOCUS       pET-21(+).        5369 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of genes with bacterial 
            translation signals.
ACCESSION   .
VERSION     .
KEYWORDS    pET-21(+).
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    158..203
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     protein_bind    complement(210..234)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(235..253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        562..639
                     /label=lacI promoter
     CDS             640..1719
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1735..1756
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2531..2719
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2824..2966
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3152..3740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3914..4771)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4772..4876)
                     /label=AmpR promoter
     rep_origin      complement(4903..5358)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tcctagaggg gaattgttat ccgctcacaa ttcccctata
      241 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
      301 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      361 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      421 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      481 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      541 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
      601 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
      661 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
      721 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
      781 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
      841 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
      901 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
      961 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     1021 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     1081 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     1141 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     1201 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     1261 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     1321 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     1381 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     1441 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     1501 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     1561 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     1621 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1741 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc
     1801 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     1861 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     1921 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     1981 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     2041 cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     2101 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     2161 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     2221 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     2281 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     2341 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
     2401 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     2461 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
     2521 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     2581 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
     2641 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
     2701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     2881 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat
     2941 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     3001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4201 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     4261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata
     4921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     4981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     5161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     5221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     5281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     5341 cgccgctaca gggcgcgtcc cattcgcca
//
