LOCUS       pAcGP67-B.        9765 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for secreting expressed proteins using 
            the GP64 signal sequence. For other reading frames, use pAcGP67-A or
            pAcGP67-C.
ACCESSION   .
VERSION     .
KEYWORDS    pAcGP67-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4189..4248
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     misc_feature    4258..4318
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4780..6235
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7146..7162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7170..7186)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7194..7224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7239..7260)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7548..8136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8310..9167)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9168..9272)
                     /label=AmpR promoter
     primer_bind     9746..9762
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcttgga
     4261 tcccgggcca tgggaattcc ggagcggccg ctgcagatct gatcctttcc tgggacccgg
     4321 caagaaccaa aaactcactc tcttcaagga aatccgtaat gttaaacccg acacgatgaa
     4381 gcttgtcgtt ggatggaaag gaaaagagtt ctacagggaa acttggaccc gcttcatgga
     4441 agacagcttc cccattgtta acgaccaaga agtgatggat gttttccttg ttgtcaacat
     4501 gcgtcccact agacccaacc gttgttacaa attcctggcc caacacgctc tgcgttgcga
     4561 ccccgactat gtacctcatg acgtgattag gatcgtcgag ccttcatggg tgggcagcaa
     4621 caacgagtac cgcatcagcc tggctaagaa gggcggcggc tgcccaataa tgaaccttca
     4681 ctctgagtac accaactcgt tcgaacagtt catcgatcgt gtcatctggg agaacttcta
     4741 caagcccatc gtttacatcg gtaccgactc tgctgaagag gaggaaattc tccttgaagt
     4801 ttccctggtg ttcaaagtaa aggagtttgc accagacgca cctctgttca ctggtccggc
     4861 gtattaaaac acgatacatt gttattagta catttattaa gcgctagatt ctgtgcgttg
     4921 ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg
     4981 gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat
     5041 taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc
     5101 cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc
     5161 ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc
     5221 gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa
     5281 ttgactcgac gtaaacacgt taaataaagc ttggacatat ttaacatcgg gcgtgttagc
     5341 tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga
     5401 cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa
     5461 atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa
     5521 tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg
     5581 taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac
     5641 catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga
     5701 tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat
     5761 tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc gatttttttc
     5821 gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc
     5881 cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg gtggtggagg
     5941 cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta taatttgttc
     6001 tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg gctgcacaac
     6061 ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat tataattgga
     6121 atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta ccgtgccgat
     6181 atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca agctccgcac
     6241 gccgataaca agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc
     6301 gtcgacgtac atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta aaatatttaa
     6361 aagaacatct ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga taatttgcgc
     6421 ttccgcagta tcgacacgtt caaaaaattg atgcgcatca attttgttgt tcctattatt
     6481 gaataaataa gattgtacag attcatatct acgattcgtc atggccacca caaatgctac
     6541 gctgcaaacg ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc ggtataaaat
     6601 aatcaacggg cgctttggca aaatatctat tttatcgcac aagcccacta gcaaattgta
     6661 tttgcagaaa acaatttcgg cgcacaattt taacgctgac gaaataaaag ttcaccagtt
     6721 aatgagcgac cacccaaatt ttataaaaat ctattttaat cacggttcca tcaacaacca
     6781 agtgatcgtg atggactaca ttgactgtcc cgatttattt gaaacactac aaattaaagg
     6841 cgagctttcg taccaacttg ttagcaatat tattagacag ctgtgtgaag cgctcaacga
     6901 tttgcacaag cacaatttca tacacaacga cataaaactc gaaaatgtct tatatttcga
     6961 agcacttgat cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa actcacttag
     7021 cgtgcacgac ggcacgttgg agtattttag tccggaaaaa attcgacaca caactatgca
     7081 cgtttcgttt gactggtacg cggcgtgtta acatacaagt tgctaaccgg cggttcgtaa
     7141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7981 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9301 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9361 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     9421 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     9481 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     9541 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     9601 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9661 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9721 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgcc
//
