LOCUS       pAcG2T.        8530 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing N-terminally GST-tagged 
            proteins with a thrombin cleavage site, for use with the BD 
            BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcG2T.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2866..2883
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_recomb     2923..4280
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(5424..5440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5653..6108
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6390..6494
                     /label=AmpR promoter
     CDS             6495..7352
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7526..8114
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8402..8423
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8438..8468
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8476..8492
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8500..8516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
     2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
     2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
     2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
     2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
     2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
     2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
     2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
     2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
     2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
     2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
     2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctggt tccgcgtgga
     2881 tccccgggaa ttcatcgtga ctgactgacg atctgagatc cggttattag tacatttatt
     2941 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
     3001 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
     3061 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
     3121 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
     3181 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
     3241 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
     3301 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
     3361 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
     3421 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
     3481 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
     3541 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
     3601 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
     3661 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
     3721 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
     3781 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
     3841 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
     3901 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
     3961 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
     4021 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
     4081 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
     4141 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
     4201 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
     4261 gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact
     4321 acagcattgt agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca
     4381 aaaacgtcgt tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg
     4441 ttgtcgtaaa tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat
     4501 tgatgcgcat caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat
     4561 ctacgattcg tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa
     4621 aactgcaaaa acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct
     4681 attttatcgc acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat
     4741 tttaacgctg acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa
     4801 atctatttta atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt
     4861 cccgatttat ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat
     4921 attattagac agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac
     4981 gacataaaac tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat
     5041 tacggattgt gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt
     5101 agtccggaaa aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt
     5161 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
     5221 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
     5281 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
     5341 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
     5401 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5581 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5641 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5701 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5761 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5821 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     5881 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5941 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     6001 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6061 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8401 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8521 attacgaatt
//
