LOCUS       pALTER-MAX.        5538 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector with the CMV enhancer/promoter and with 
            ampicillin and chloramphenicol resistance markers.
ACCESSION   .
VERSION     .
KEYWORDS    pALTER-MAX.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The AmpR gene was edited to correct an apparent error in the GenBank
            sequence.
FEATURES             Location/Qualifiers
     source          1..5538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1091..1137
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1145..1162)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1180..1301)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1487..1942
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2014..2371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2520..2622
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2623..3279
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     polyA_signal    3379..3427
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3741..3845
                     /label=AmpR promoter
     CDS             3846..4703
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4877..5465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcaaa
     1141 gttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt gatgagtttg
     1201 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1321 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     1381 acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg aagaggcccg
     1441 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     1501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc acgccacgtt cgccggcttt
     1621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1921 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     1981 tttcacaccg catacgcgga tcttccgtac cttctgaggc ggaaagaacc agctgtggaa
     2041 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2101 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     2341 aggctttttt ggaggcctag gcttttgcaa aaagcttgat tcttctgaca caacagtctc
     2401 gaacttaagg ctagcgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
     2461 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa gtgagacgtt
     2521 gatcggcacg taagaggttc caactttcac cataatcaaa taagatcact accgggcgta
     2581 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2641 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2701 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2761 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2821 atgaatgctc atccggaact ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2881 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2941 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     3001 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     3061 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     3121 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     3181 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     3241 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
     3301 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
     3361 agaaattcgt cgaagcgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     3421 ttgtgtgaat cgatagcgat aaggatcgga agatccgcgt atggtgcact ctcagtacaa
     3481 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4081 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4381 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4561 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5521 acatggctcg acagatct
//
