LOCUS       pAB-bee-8xHis.        9965 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of secreted 
            proteins with N- and C-terminal 8xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-bee-8xHis.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4143..4205
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             4209..4232
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             4451..4474
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_recomb     4980..6435
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(7394..7424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7439..7460)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8510..9367)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9368..9472)
                     /label=AmpR promoter
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatcta
     4141 aaatgaaatt cttagtcaac gttgcccttg tttttatggt cgtatacatt tcttacatct
     4201 atgcggccca tcaccaccat caccaccatc acggagcggc cgctccggaa ttctagaagg
     4261 tacccgggat cccactgaag atgatgacat gaagatgaag atgatgatgg tgatgacatc
     4321 gatgccgatg atgacgacga cgatgataat gataattctg atgaggataa tgaagacagt
     4381 gatgatgacg atgatgacga tgaatagctc cagccctggg tgattctgat gagtgacaga
     4441 attcgtcgag caccaccacc accaccacca ccactaattg attaatacct aggtcgacgc
     4501 gatcctttcc tgggacccgg caagaaccaa aaactcactc tcttcaagga aatccgtaat
     4561 gttaaacccg acacgatgaa gcttgtcgtt ggatggaaag gaaaagagtt ctacagggaa
     4621 acttggaccc gcttcatgga agacagcttc cccattgtta acgaccaaga agtgatggat
     4681 gttttccttg ttgtcaacat gcgtcccact agacccaacc gttgttacaa attcctggcc
     4741 caacacgctc tgcgttgcga ccccgactat gtacctcatg acgtgattag gatcgtcgag
     4801 ccttcatggg tgggcagcaa caacgagtac cgcatcagcc tggctaagaa gggcggcggc
     4861 tgcccaataa tgaaccttca ctctgagtac accaactcgt tcgaacagtt catcgatcgt
     4921 gtcatctggg agaacttcta caagcccatc gtttacatcg gtaccgactc tgctgaagag
     4981 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca
     5041 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa
     5101 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     5161 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     5221 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     5281 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     5341 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     5401 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     5461 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat
     5521 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     5581 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     5641 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
     5701 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
     5761 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
     5821 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
     5881 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
     5941 acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg
     6001 agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa
     6061 ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat
     6121 ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt
     6181 gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca
     6241 ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc
     6301 aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg
     6361 ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga
     6421 gattgtctca agctccgcac gccgataaca agccttttca tttttactac agcattgtag
     6481 tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa aacgtcgttg
     6541 gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt gtcgtaaatg
     6601 ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg atgcgcatca
     6661 attttgttgt tcctattatt gaataaataa gattgtacag attcatatct acgattcgtc
     6721 atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa ctgcaaaaac
     6781 gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat tttatcgcac
     6841 aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt taacgctgac
     6901 gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat ctattttaat
     6961 cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc cgatttattt
     7021 gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat tattagacag
     7081 ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga cataaaactc
     7141 gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta cggattgtgc
     7201 aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag tccggaaaaa
     7261 attcgacaca caactatgca cgtttcgttt gactggtacg cggcgtgtta acatacaagt
     7321 tgctaaccgg cggttcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9541 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9721 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9781 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     9841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9961 gtgcc
//
