LOCUS       pAB-GST.       10335 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of proteins 
            with an HRV 3C-cleavable N-terminal GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-GST.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4138..4791
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             4798..4821
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    4825..4888
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     5350..6805
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(7764..7794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7809..7830)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8118..8706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8880..9737)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9738..9842)
                     /label=AmpR promoter
     primer_bind     10316..10332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctaaaatg
     4141 agccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg
     4201 gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg
     4261 cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt
     4321 gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg
     4381 ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat
     4441 attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat
     4501 tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca
     4561 tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt
     4621 gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa
     4681 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg
     4741 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa atcggatctg
     4801 gaagttctgt tccaggggcc cctgggatcc ccggaattcc cgggtcgact cgagcggccg
     4861 ctgcagatct gatcctttcc tgggacccgg caagaaccaa aaactcactc tcttcaagga
     4921 aatccgtaat gttaaacccg acacgatgaa gcttgtcgtt ggatggaaag gaaaagagtt
     4981 ctacagggaa acttggaccc gcttcatgga agacagcttc cccattgtta acgaccaaga
     5041 agtgatggat gttttccttg ttgtcaacat gcgtcccact agacccaacc gttgttacaa
     5101 attcctggcc caacacgctc tgcgttgcga ccccgactat gtacctcatg acgtgattag
     5161 gatcgtcgag ccttcatggg tgggcagcaa caacgagtac cgcatcagcc tggctaagaa
     5221 gggcggcggc tgcccaataa tgaaccttca ctctgagtac accaactcgt tcgaacagtt
     5281 catcgatcgt gtcatctggg agaacttcta caagcccatc gtttacatcg gtaccgactc
     5341 tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc
     5401 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta
     5461 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt
     5521 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt
     5581 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt
     5641 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt
     5701 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt
     5761 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt
     5821 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc
     5881 ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca
     5941 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt
     6001 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc
     6061 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa
     6121 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg
     6181 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg
     6241 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc
     6301 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt
     6361 gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct
     6421 gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc
     6481 agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg
     6541 tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg
     6601 caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg
     6661 gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat
     6721 tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt
     6781 attgtaaaga gattgtctca agctccgcac gccgataaca agccttttca tttttactac
     6841 agcattgtag tggcgagaca cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa
     6901 aacgtcgttg gcaagcttta aaatatttaa aagaacatct ctgttcagca ccactgtgtt
     6961 gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta tcgacacgtt caaaaaattg
     7021 atgcgcatca attttgttgt tcctattatt gaataaataa gattgtacag attcatatct
     7081 acgattcgtc atggccacca caaatgctac gctgcaaacg ctggtacaat tttacgaaaa
     7141 ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg cgctttggca aaatatctat
     7201 tttatcgcac aagcccacta gcaaattgta tttgcagaaa acaatttcgg cgcacaattt
     7261 taacgctgac gaaataaaag ttcaccagtt aatgagcgac cacccaaatt ttataaaaat
     7321 ctattttaat cacggttcca tcaacaacca agtgatcgtg atggactaca ttgactgtcc
     7381 cgatttattt gaaacactac aaattaaagg cgagctttcg taccaacttg ttagcaatat
     7441 tattagacag ctgtgtgaag cgctcaacga tttgcacaag cacaatttca tacacaacga
     7501 cataaaactc gaaaatgtct tatatttcga agcacttgat cgcgtgtatg tttgcgatta
     7561 cggattgtgc aaacacgaaa actcacttag cgtgcacgac ggcacgttgg agtattttag
     7621 tccggaaaaa attcgacaca caactatgca cgtttcgttt gactggtacg cggcgtgtta
     7681 acatacaagt tgctaaccgg cggttcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7741 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7801 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7861 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7921 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9181 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9481 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9781 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9841 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9901 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     9961 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10021 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10081 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10141 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    10201 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10261 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10321 acgacggcca gtgcc
//
