根据引物模拟PCR产物

PCR Products accepts one or more DNA sequence templates and two primer sequences. The program searches for perfectly matching primer annealing sites that can generate a PCR product. Any resulting products are sorted by size, and they are given a title specifying their length, their position in the original sequence, and the primers that produced them. You can use linear or circular molecules as the template. Use PCR Products to determine the product sizes you can expect to see when you perform PCR in the lab.

在下面的文本框中输入原始序列或者一条或者多条FASTA序列。 Input limit is 200000 characters.


Enter the name of the first primer, followed by its sequence in the 5' to 3' direction. Degenerate sites can be represented using IUPAC DNA characters.

Enter the name of the second primer, followed by its sequence in the 5' to 3' direction. Degenerate sites can be represented using IUPAC DNA characters.

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  • Treat sequences as molecules.