载体基本信息 | |||
载体名称 | LITMUS39 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2817 bp | 载体类型 | Cloning Vectors |
载体来源 | Evans PD, Cook SN, Riggs PD, Noren CJ. | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
LITMUS39 载体载体图谱
LITMUS39 载体载体序列
LOCUS LITMUS39. 2817 bp DNA circular SYN 01-JAN-1980 DEFINITION High copy-number E. coli vector with a multiple cloning site flanked by mutant T7 promoters. The MCS is reversed relative to LITMUS38. ACCESSION . VERSION . KEYWORDS LITMUS39. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2817) AUTHORS Evans PD, Cook SN, Riggs PD, Noren CJ. TITLE LITMUS: multipurpose cloning vectors with a novel system for bidirectional in vitro transcription. JOURNAL BioTechniques 1995;19:130-5. PUBMED 7669286 REFERENCE 2 (bases 1 to 2817) AUTHORS New England Biolabs TITLE Direct Submission REFERENCE 3 (bases 1 to 2817) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "1995"; volume: "19"; pages: "130-5" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..2817 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 38..142 /label=AmpR promoter CDS 143..1000 /label=AmpR /note="beta-lactamase" rep_origin complement(1045..1554) /direction=LEFT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin 1665..2253 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2314..2335 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2350..2380 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2388..2404 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2412..2428 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2449..2466 /gene="lacZalpha" /label=lacZalpha promoter /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" misc_feature 2460..2602 /gene="lacZalpha" /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(2597..2614) /note="T7 promoter (mutant)" /note="mutant version of the promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2618..2634) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
我们不提供该载体,载体信息仅供查询和参考。