- 载体名称:
- pcDNA3.1(+)
- 载体抗性:
- Ampicillin
- 载体长度:
- 5428 bp
- 载体类型:
- Mammalian Expression Vectors
- 复制子:
- ori
- 载体来源:
- Invitrogen (Life Technologies)
- 筛选标记:
- Neomycin/G418(Geneticin)
- 拷贝数:
- High copy number
- 启动子:
- SV40
- 3'引物:
- M13 rev
pcDNA3.1(+) 载体载体图谱
pcDNA3.1(+) 载体载体序列
LOCUS pcDNA3.1(+). 5428 bp DNA circular SYN 01-JAN-1980
DEFINITION Mammalian expression vector with the CMV promoter. The MCS is in the
forward (+) orientation.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1(+).
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5428)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5428)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT The cloned gene must include a Kozak sequence and start codon.
FEATURES Location/Qualifiers
source 1..5428
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..1002
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 1028..1252
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1298..1726
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1740..2069
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2136..2927
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3104..3237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3274..3290)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3298..3314)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3322..3352)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3367..3388)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3676..4261)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4435..5292)
/label=AmpR
/note="beta-lactamase"
promoter complement(5293..5397)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。