下载GeneBank文件(.gb)

LOCUS       lentiCas9-Blast.       12859 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Zhang lab lentiviral vector with a blasticidin resistance marker, 
            also known as pLentiCas9-Blast, for expressing Cas9. Use together 
            with lentiGuide-Puro.
ACCESSION   .
VERSION     .
KEYWORDS    lentiCas9-Blast.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Sanjana NE, Shalem O, Zhang F.
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat. Methods 2014;11:783-4.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 12859)
  AUTHORS   Zhang Lab / Addgene #52962
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2014"; volume: "11"; pages: "783-4"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2886..6989
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             6990..7037
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from
                     nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7038..7061
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             7071..7127
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             7128..7523
                     /label=BSD
                     /note="blasticidin S deaminase"
     misc_feature    7551..8139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8664..8844
                     /label=3' LTR (truncated)
                     /note="3' LTR (truncated)"
                     /note="truncated long terminal repeat (LTR) from HIV-1"
     polyA_signal    8876..9100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9146..9574
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9588..9917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        9965..10012
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             10031..10402
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    10535..10668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(10753..10783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10798..10819)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11107..11695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11869..12726)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12727..12831)
                     /label=AmpR promoter