载体基本信息 | |||
载体名称 | pEGFP-C1 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 4731 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Clontech | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number | 启动子 | CMV |
克隆方法 | Enzyme Cut | 表达方法 | Transient |
pEGFP-C1 载体载体图谱
pEGFP-C1 载体载体序列
LOCUS Exported 4731 bp ds-DNA circular SYN 26-11-2013 DEFINITION Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP-C2 or pEGFP-C3. ACCESSION . VERSION . KEYWORDS pEGFP-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4731) AUTHORS Clontech TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..4731 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 61..364 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 613..1329 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 1330..1395 /note="MCS" /note="multiple cloning site of fluorescent protein plasmids" misc_feature 1404..1414 /note="stop codons" /note="stop codons in all three reading frames" polyA_signal 1519..1640 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1647..2102) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2129..2233 /gene="bla" /note="AmpR promoter" promoter 2235..2592 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2443..2578 /note="SV40 ori" /note="SV40 origin of replication" CDS 2627..3421 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 3653..3700 /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 4029..4617 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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