载体基本信息 | |||
载体名称 | pFusionRed-B | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4129 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Evrogen | 拷贝数 | High copy number |
pFusionRed-B 载体载体图谱
pFusionRed-B 载体载体序列
LOCUS pFusionRed-B. 4129 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing the monomeric red fluorescent protein FusionRed in bacteria. ACCESSION . VERSION . KEYWORDS pFusionRed-B. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4129) AUTHORS Evrogen TITLE Direct Submission REFERENCE 2 (bases 1 to 4129) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4129 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..54 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 62..78 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 114..116 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 147..842 /label=FusionRed /note="monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)" terminator 875..969 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 1013..1669 /label=CmR /note="chloramphenicol acetyltransferase" terminator 1737..1823 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 1979..2119 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(2305..2893) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3067..3924) /label=AmpR /note="beta-lactamase" promoter complement(3925..4029) /label=AmpR promoter
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