| 载体基本信息 | |||
| 载体名称 | pAd/PL-DEST | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 34864 bp | 载体类型 | Gateway Cloning Vectors |
| 载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pAd/PL-DEST 载体载体图谱
pAd/PL-DEST 载体载体序列
LOCUS pAd_PL-DEST. 34864 bp DNA circular UNA 01-JAN-1980
DEFINITION Promoterless adenoviral Gateway(R) destination vector for inserting
a gene expression cassette.
ACCESSION .
VERSION .
KEYWORDS pAd PL-DEST.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 34864)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 34864)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..34864
/mol_type="genomic DNA"
/organism="synthetic DNA construct"
repeat_region 1..103
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
protein_bind 512..636
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 661..691
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 745..1401
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 1746..2048
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2092..2216)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 2330..2749
/gene="IX"
/label=IX
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(2815..4149)
/gene="IVa2"
/label=IVa2
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
CDS complement(3921..7502)
/gene="POL"
/label=POL
/note="DNA polymerase from Human adenovirus C serotype 2.
Accession#: P03261"
CDS complement(7307..9310)
/gene="PTP"
/label=PTP
/note="Preterminal protein from Human adenovirus C serotype
5. Accession#: P04499"
CDS 9771..11015
/gene="L1"
/label=L1
/note="Packaging protein 3 from Human adenovirus C serotype
5. Accession#: P04496"
CDS 11039..12793
/note="Pre-hexon-linking protein IIIa from Human adenovirus
C serotype 5. Accession#: P12537"
CDS 12877..14589
/gene="L2"
/label=L2
/note="Penton protein from Human adenovirus C serotype 5.
Accession#: P12538"
CDS 14599..15192
/gene="L2"
/label=L2
/note="Pre-histone-like nucleoprotein from Human adenovirus
C serotype 2. Accession#: P68950"
CDS 15265..16368
/gene="L2"
/label=L2
/note="Core-capsid bridging protein from Human adenovirus C
serotype 5. Accession#: P24938"
CDS 16399..16638
/note="Pre-core protein X from Human adenovirus C serotype
5. Accession#: Q2KS10"
CDS 16724..17473
/gene="L3"
/label=L3
/note="Pre-protein VI from Human adenovirus C serotype 5.
Accession#: P24937"
CDS 17563..20418
/gene="L3"
/label=L3
/note="Hexon protein from Human adenovirus C serotype 5.
Accession#: P04133"
CDS 20454..21065
/gene="L3"
/label=L3
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(21167..22753)
/gene="DBP"
/label=DBP
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 22782..25202
/gene="L4"
/label=L4
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 25895..26575
/gene="L4"
/label=L4
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS 27885..29627
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
CDS complement(30039..30920)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
CDS complement(31199..31546)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
CDS complement(31987..32370)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
repeat_region 32680..32782
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(33006..33594)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(33749..34606)
/label=AmpR
/note="beta-lactamase"
promoter complement(34607..34711)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。