载体基本信息 | |||
载体名称 | pcDNA6.2/C-YFP-DEST | 载体抗性 | Ampicillin |
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载体长度 | 7442 bp | 载体类型 | Gateway Cloning Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pcDNA6.2/C-YFP-DEST 载体载体图谱
pcDNA6.2/C-YFP-DEST 载体载体序列
LOCUS Exported 7442 bp ds-DNA circular SYN 18-11-2013 DEFINITION Vivid Colors(TM) Gateway(R) destination vector for expressing C-terminally YFP-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA6.2 C-YFP-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7442) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission FEATURES Location/Qualifiers source 1..7442 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 105..484 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 485..688 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 733..751 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 785..909 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR1" /note="recombination site for the Gateway(R) LR reaction" promoter 934..964 /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" CDS 1018..1677 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 2019..2324 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(2365..2489) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" CDS 2503..3222 /codon_start=1 /product="enhanced yellow variant of GFP" /note="Topaz YFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 3294..3342 /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 3544..3972 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3986..4315 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 4166..4301 /note="SV40 ori" /note="SV40 origin of replication" promoter 4363..4410 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 4429..4827 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /note="BSD" /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 4985..5106 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(5557..6145) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6316..7176) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7177..7281) /gene="bla" /note="AmpR promoter"
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