载体基本信息 | |||
载体名称 | pBluescript-FL | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2985 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescript-FL 载体载体图谱
pBluescript-FL 载体载体序列
LOCUS 40924_6766 2985 bp DNA circular SYN 01-JAN-1980 DEFINITION cDNA cloning vector derived from pBluescript II KS(-) by replacing the SmaI site with a fragment containing two PflMI sites. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2985) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission REFERENCE 2 (bases 1 to 2985) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2985 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 4..459 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 677..693 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(751..767) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(797..815) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(836..852) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(860..876) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(884..914) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(929..950) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1238..1826) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2000..2857) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2858..2962) /label=AmpR promoter
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