载体基本信息 | |||
载体名称 | pCMV SPORT | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4380 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
pCMV SPORT 载体载体图谱
pCMV SPORT 载体载体序列
LOCUS Exported 4380 bp ds-DNA circular SYN 28-2-2013 DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs. ACCESSION . VERSION . KEYWORDS pCMV SPORT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4380) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission FEATURES Location/Qualifiers source 1..4380 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" polyA_signal 125..259 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 646..664 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 682..779 /note="MCS" /note="multiple cloning site" promoter complement(787..805) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" promoter complement(921..1124) /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" enhancer 1125..1428 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" protein_bind 1458..1474 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1482..1513) /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" rep_origin complement(1975..2563) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2641..2674 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(2776..3636) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(3924..4379) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
我们不提供该载体,载体信息仅供查询和参考。