pCMV SPORT 载体 (V011745#)

载体基本信息
载体名称 pCMV SPORT 载体抗性 Ampicillin
载体长度 4380 bp 载体类型 I.M.A.G.E. Consortium Plasmids
载体来源 I.M.A.G.E. Consortium 拷贝数 High copy number

pCMV SPORT 载体载体图谱

pCMV SPORT4380 bp600120018002400300036004200SV40 poly(A) signalT7 promoterMCSSP6 promoterCMV promoterCMV enhancerlac operatorlac UV5 promoteroriloxPAmpRf1 ori

pCMV SPORT 载体载体序列

LOCUS       Exported                4380 bp ds-DNA    circular SYN 28-2-2013
DEFINITION  Vector for cloning and transient mammalian cell expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV SPORT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     polyA_signal    125..259
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        646..664
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    682..779
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(787..805)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(921..1124)
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1125..1428
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    1458..1474
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1482..1513)
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     rep_origin      complement(1975..2563)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2641..2674
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(2776..3636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3924..4379)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"

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