载体基本信息 | |||
载体名称 | pCMV SPORT2 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4474 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
启动子 | CMV | 5'引物 | M13 fwd |
3'引物 | M13 rev |
pCMV SPORT2 载体载体图谱
pCMV SPORT2 载体载体序列
LOCUS pCMV_SPORT2. 4474 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs. ACCESSION . VERSION . KEYWORDS pCMV SPORT2. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4474) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission REFERENCE 2 (bases 1 to 4474) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4474 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(125..259) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind 664..680 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 691..709 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 727..824 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(832..850) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(873..889) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter complement(1015..1218) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" enhancer complement(1219..1522) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" protein_bind complement(1552..1568) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1576..1607) /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" rep_origin complement(2069..2657) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind complement(2735..2768) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." CDS complement(2873..3730) /label=AmpR /note="beta-lactamase" rep_origin complement(4018..4473) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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