载体基本信息 | |||
载体名称 | pCMV SPORT4 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4387 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pCMV SPORT4 载体载体图谱
pCMV SPORT4 载体载体序列
LOCUS Exported 4387 bp ds-DNA circular SYN 28-2-2013 DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs. ACCESSION . VERSION . KEYWORDS pCMV SPORT4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4387) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission FEATURES Location/Qualifiers source 1..4387 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" polyA_signal 125..259 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind 664..680 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 691..709 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 727..824 /note="MCS" /note="multiple cloning site" promoter complement(832..850) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(873..889) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter complement(1015..1218) /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" enhancer 1219..1522 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" rep_origin complement(1982..2570) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2648..2681 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(2783..3643) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(3931..4386) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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