- 载体名称:
- pAB-6xHis-MBP
- 载体抗性:
- Ampicillin
- 载体长度:
- 10833 bp
- 载体类型:
- Insect Cell Vectors
- 复制子:
- ori
- 载体来源:
- AB Vector
- 拷贝数:
- High copy number
- 启动子:
- polyhedrin
pAB-6xHis-MBP 载体载体图谱
pAB-6xHis-MBP 载体载体序列
LOCUS pAB-6xHis-MBP. 10833 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid for high-level expression of proteins with a thrombin-cleavable N-terminal 6xHis-MBP tag. ACCESSION . VERSION . KEYWORDS pAB-6xHis-MBP. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10833) AUTHORS AB Vector TITLE Direct Submission REFERENCE 2 (bases 1 to 10833) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..10833 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 76..579 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2662..3291 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3170..3997 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 4001..4092 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 4138..4140 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 4147..4164 /label=6xHis /note="6xHis affinity tag" CDS 4192..5283 /label=MBP /note="maltose binding protein from E. coli" CDS 5302..5319 /label=thrombin site /note="thrombin recognition and cleavage site" misc_recomb 5848..7303 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" promoter complement(8262..8292) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8307..8328) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8616..9204) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9378..10235) /label=AmpR /note="beta-lactamase" promoter complement(10236..10340) /label=AmpR promoter primer_bind 10814..10830 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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