- 载体名称:
- pAB-6xHis
- 载体抗性:
- Ampicillin
- 载体长度:
- 9698 bp
- 载体类型:
- Insect Cell Vectors
- 复制子:
- ori
- 载体来源:
- AB Vector
- 拷贝数:
- High copy number
- 启动子:
- polyhedrin
pAB-6xHis 载体载体图谱
pAB-6xHis 载体载体序列
LOCUS pAB-6xHis. 9698 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid for high-level expression of proteins with a thrombin-cleavable N-terminal 6xHis tag. ACCESSION . VERSION . KEYWORDS pAB-6xHis. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9698) AUTHORS AB Vector TITLE Direct Submission REFERENCE 2 (bases 1 to 9698) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9698 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 76..579 /gene="PTP" /label=PTP /note="Tyrosine-protein phosphatase from Autographa californica nuclear polyhedrosis virus. Accession#: P24656" CDS 2662..3291 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 3170..3997 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 4001..4092 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 4138..4140 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 4147..4164 /label=6xHis /note="6xHis affinity tag" CDS 4174..4191 /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 4192..4251 /label=MCS /note="MCS" /note="multiple cloning site" misc_recomb 4713..6168 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" primer_bind complement(7079..7095) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7103..7119) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7127..7157) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7172..7193) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7481..8069) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8243..9100) /label=AmpR /note="beta-lactamase" promoter complement(9101..9205) /label=AmpR promoter primer_bind 9679..9695 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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