pAcAB3 载体 (V011693#)

载体基本信息
载体名称 pAcAB3 载体抗性 Ampicillin
载体长度 10096 bp 载体类型 Insect Cell Vectors
载体来源 AB Vector 拷贝数 High copy number

pAcAB3 载体载体图谱

pAcAB310096 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000PTPLEF-2SV40 poly(A) signalp10 promoterpolyhedrin promoterp10 promotercontains part of ORF1629AmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

pAcAB3 载体载体序列

LOCUS       pAcAB3.       10096 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of up to 
            three proteins simultaneously in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    pAcAB3.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..578
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2661..3290
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3169..3996
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(4000..4133)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4462..4571)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(4722..4813)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4839..4948
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_recomb     4967..6324
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        7960..8064
                     /label=AmpR promoter
     CDS             8065..8922
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9096..9684
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9972..9993
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10008..10038
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10046..10062
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10070..10086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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