下载GeneBank文件(.gb)

LOCUS       pAcGHLT_B.        8755 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing proteins with an 
            N-terminal GST-6xHis-PKA-thrombin cassette. For other reading 
            frames, use pAcGHLT A or pAcGHLT C.
ACCESSION   .
VERSION     .
KEYWORDS    pAcGHLT B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2875..2892
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             2899..2916
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label=recognition site for phosphorylation by protein
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2926..2943
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_feature    2966..3027
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     3047..4505
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(5649..5665)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5878..6333
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6615..6719
                     /label=AmpR promoter
     CDS             6720..7577
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7751..8339
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8627..8648
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8663..8693
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8701..8717
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8725..8741
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"