下载GeneBank文件(.gb)

LOCUS       pFastBac_Dual.        5238 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Dual-promoter vector for co-expression of two proteins in insect 
            cells using the Bac-to-Bac(R) baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pFastBac Dual.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Transform into DH10Bac(TM) E. coli cells.
FEATURES             Location/Qualifiers
     source          1..5238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        584..688
                     /label=AmpR promoter
     CDS             689..1546
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1720..2308
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2611..2835)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2905..3435)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3624..3652)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    complement(4161..4209)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     misc_feature    4274..4337
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(4345..4454)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4478..4569
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4606..4704
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    4828..4962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4991..5156)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"