| 载体基本信息 | |||
| 载体名称 | pFastBac™1 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 4776 bp | 载体类型 | Insect Cell Vectors |
| 载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pFastBac™1载体质粒图谱
pFastBac™1载体载体序列
LOCUS Exported 4776 bp ds-DNA circular SYN 04-4-2014
DEFINITION Vector for high-level expression of proteins in insect cells using
the Bac-to-Bac(R) baculovirus system.
ACCESSION .
VERSION .
KEYWORDS pFastBac1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4776)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
COMMENT Transform into DH10Bac(TM) E. coli cells.
FEATURES Location/Qualifiers
source 1..4776
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
rep_origin 2..457
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 484..588
/gene="bla"
/note="AmpR promoter"
CDS 589..1449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1620..2208
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
mobile_element 2511..2735
/mobile_element_type="transposon:Tn7"
/note="Tn7R"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
CDS complement(2802..3335)
/codon_start=1
/gene="aacC1"
/allele="
"
/product="gentamycin acetyltransferase"
/note="GmR"
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(3524..3552)
/gene="intI1 (promoter lies within the coding sequence)"
/note="Pc promoter"
/note="class 1 integron promoter"
promoter 3904..3995
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
misc_feature 4032..4142
/note="MCS"
/note="multiple cloning site"
polyA_signal 4266..4400
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
mobile_element 4429..4594
/mobile_element_type="transposon:Tn7"
/note="Tn7L"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg tcgacgagct
4081 cactagtcgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
4141 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
4201 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
4261 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
4321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
4381 tatcttatca tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt
4441 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
4501 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
4561 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
4621 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
4681 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
4741 atatcaacgc ttatttgcag cctgaatggc gaatgg
//
pFastBac™1载体载体特征
| 一级属性 | 二级属性 | 属性值 | 起始位置 |
| 常规 | 长度 | 4776 | |
| 常规 | 描述 | Vector for high-level expression of proteins in insect cells using the Bac-to-Bac(R) baculovirus system. | |
| 常规 | 是否环形 | 1 | |
| 常规 | 作者 | Invitrogen (Life Technologies) | |
| 常规 | 注释 | ||
| 来源 | 实验宿主 | Insect Cells | 1..4776 |
| 来源 | 分子类型 | other DNA | 1..4776 |
| 来源 | 种属 | synthetic DNA construct | 1..4776 |
| 复制起点 | 方向 | RIGHT | 2..457 |
| 复制起点 | 备注 | f1 ori,f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis | 2..457 |
| 启动子 | 基因 | bla | 484..588 |
| 启动子 | 备注 | AmpR promoter | 484..588 |
| 编码区 | 翻译起始位点 | 1 | 589..1449 |
| 编码区 | 基因 | bla | 589..1449 |
| 编码区 | 备注 | AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics | 589..1449 |
| 编码区 | 产物 | beta-lactamase | 589..1449 |
| 编码区 | 翻译序列 | MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW | 589..1449 |
| 复制起点 | 方向 | RIGHT | 1620..2208 |
| 复制起点 | 备注 | ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication | 1620..2208 |
| 可动元件 | 可动元件类型 | transposon:Tn7 | 2511..2735 |
| 可动元件 | 备注 | Tn7R,mini-Tn7 element (right end of the Tn7 transposon) | 2511..2735 |
| 编码区 | 等位基因 | 2802..3335 | |
| 编码区 | 翻译起始位点 | 1 | 2802..3335 |
| 编码区 | 基因 | aacC1 | 2802..3335 |
| 编码区 | 备注 | GmR,confers resistance to gentamycin | 2802..3335 |
| 编码区 | 产物 | gentamycin acetyltransferase | 2802..3335 |
| 编码区 | 翻译序列 | MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT | 2802..3335 |
| 启动子 | 基因 | intI1 (promoter lies within the coding sequence) | 3524..3552 |
| 启动子 | 备注 | Pc promoter,class 1 integron promoter | 3524..3552 |
| 启动子 | 基因 | polh from Autographa californica | 3904..3995 |
| 启动子 | 备注 | polyhedrin promoter,promoter for the baculovirus polyhedrin gene | 3904..3995 |
| 其它特征区 | 备注 | MCS,multiple cloning site | 4032..4142 |
| polyA信号 | 备注 | SV40 poly(A) signal,SV40 polyadenylation signal | 4266..4400 |
| 可动元件 | 可动元件类型 | transposon:Tn7 | 4429..4594 |
| 可动元件 | 备注 | Tn7L,mini-Tn7 element (left end of the Tn7 transposon) | 4429..4594 |
我们不提供该载体，载体信息仅供查询和参考。