- 载体名称:
- pFastBac1
- 载体抗性:
- Ampicillin
- 载体长度:
- 4776 bp
- 载体类型:
- Insect Cell Vectors
- 复制子:
- ori
- 载体来源:
- Invitrogen (Life Technologies)
- 拷贝数:
- High copy number
- 启动子:
- polyhedrin
pFastBac1 载体载体图谱
pFastBac1 载体载体序列
LOCUS pFastBac1. 4776 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for high-level expression of proteins in insect cells using the Bac-to-Bac(R) baculovirus system. ACCESSION . VERSION . KEYWORDS pFastBac1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4776) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 4776) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Transform into DH10Bac(TM) E. coli cells. FEATURES Location/Qualifiers source 1..4776 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 2..457 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 484..588 /label=AmpR promoter CDS 589..1446 /label=AmpR /note="beta-lactamase" rep_origin 1620..2208 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element complement(2511..2735) /label=Tn7R /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2805..3335) /label=GmR /note="gentamycin acetyltransferase" promoter complement(3524..3552) /label=Pc promoter /note="class 1 integron promoter" promoter 3904..3995 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" misc_feature 4032..4142 /label=MCS /note="MCS" /note="multiple cloning site" polyA_signal 4266..4400 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" mobile_element complement(4429..4594) /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)"
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