载体基本信息 | |||
载体名称 | pBIND-ERα | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7605 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pBIND-ERα 载体载体图谱
pBIND-ERα 载体载体序列
LOCUS Exported 7605 bp ds-DNA circular SYN 27-7-2013 DEFINITION Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to an estrogen receptor ligand-binding domain. ACCESSION GQ229579 VERSION . KEYWORDS pBIND-ER(alpha) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7605) AUTHORS Promega TITLE Direct Submission FEATURES Location/Qualifiers source 1..7605 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 214..517 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 518..721 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" intron 857..989 /note="chimeric intron" /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1033..1051 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 1083..1520 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /note="GAL4 DNA binding domain" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV" CDS 1527..1553 /codon_start=1 /product="peptide derived from a human Ig alpha chain" /note="IgA linker" /translation="PSTPPTPSP" CDS 1563..2456 /codon_start=1 /gene="ESR1" /product="estrogen nuclear receptor alpha ligand-binding domain" /note="ER-LBD" /note=" " /protein_id=" " /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL LFAPNLLLDRNQGKCVECMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL QKYYITGEAEGFPATV" polyA_signal 2625..2746 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 2991..3348 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 3199..3334 /note="SV40 ori" /note="SV40 origin of replication" CDS 3399..4331 /codon_start=1 /product="Renilla luciferase" /note="hRluc" /note="human codon-optimized" /protein_id=" " /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" CDS 4383..5174 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5238..5286 /note="synthetic polyadenylation signal" promoter 5442..5546 /gene="bla" /note="AmpR promoter" CDS 5547..6407 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(6565..7153) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 7269..7552 /note="cer region" /note="ColE1-derived recombination site that helps to maintain plasmids as monomers"
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