载体基本信息 | |||
载体名称 | pCLuc Mini-TK 2 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6131 bp | 载体类型 | Luciferase Vectors |
载体来源 | New England Biolabs | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pCLuc Mini-TK 2 载体载体图谱
pCLuc Mini-TK 2 载体载体序列
LOCUS Exported 6131 bp ds-DNA circular SYN 03-8-2013 DEFINITION Mammalian vector with a minimal promoter for measuring promoter or enhancer activity using secreted Cypridina luciferase. ACCESSION . VERSION . KEYWORDS pCLuc Mini-TK 2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6131) AUTHORS New England Biolabs TITLE Direct Submission FEATURES Location/Qualifiers source 1..6131 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" misc_feature 20..68 /note="MCS" /note="multiple cloning site" promoter 69..131 /note="Mini-TK promoter" /note="minimal herpes simplex virus (HSV) thymidine kinase promoter" misc_signal 139..148 /note="Kozak sequence" /note=" " CDS 145..1806 /codon_start=1 /gene=" " /product="secreted Cypridina luciferase" /note="CLuc" /note="contains 30 codon substitutions for efficient translation in mammalian cells" /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK" polyA_signal 1815..1863 /note="synthetic polyadenylation signal" rep_origin 1997..2425 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2439..2769 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2620..2755 /note="SV40 ori" /note="SV40 origin of replication" CDS 2836..3630 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3804..3925 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(4376..4964) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5135..5995) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5996..6100) /gene="bla" /note="AmpR promoter"
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