载体基本信息 | |||
载体名称 | pCMV-CLuc 2 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6905 bp | 载体类型 | Luciferase Vectors |
载体来源 | New England Biolabs | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pCMV-CLuc 2 载体载体图谱
pCMV-CLuc 2 载体载体序列
LOCUS Exported 6905 bp ds-DNA circular SYN 03-8-2013 DEFINITION Control vector with a strong constitutive CMV promoter for expressing secreted Cypridina luciferase in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-CLuc 2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6905) AUTHORS New England Biolabs TITLE Direct Submission FEATURES Location/Qualifiers source 1..6905 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_signal 913..922 /note="Kozak sequence" /note=" " CDS 919..2580 /codon_start=1 /gene=" " /product="secreted Cypridina luciferase" /note="CLuc" /note="contains 30 codon substitutions for efficient translation in mammalian cells" /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK" polyA_signal 2589..2637 /note="synthetic polyadenylation signal" rep_origin 2771..3199 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3213..3543 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 3394..3529 /note="SV40 ori" /note="SV40 origin of replication" CDS 3610..4404 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 4578..4699 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(5150..5738) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5909..6769) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6770..6874) /gene="bla" /note="AmpR promoter"
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