载体基本信息 | |||
载体名称 | pGEM-luc | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4931 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pGEM-luc 载体载体图谱
pGEM-luc 载体载体序列
LOCUS Exported 4931 bp ds-DNA circular SYN 25-10月-2012 DEFINITION Plasmid carrying the firefly luciferase gene flanked by unique restriction sites. ACCESSION X65316 VERSION . KEYWORDS pGEM-luc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4931) AUTHORS Promega TITLE Direct Submission COMMENT Derived from pGEM(R)-11Zf(–). FEATURES Location/Qualifiers source 1..4931 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 9..50 /note="MCS" /note="multiple cloning site" CDS complement(102..1754) /codon_start=1 /gene="luc" /product="firefly luciferase" /EC_number=" " /note="luciferase" /experiment=" " /protein_id=" " /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" misc_feature 1757..1795 /note="MCS" /note="multiple cloning site" promoter complement(1802..1820) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1838..1854) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 1862..1878 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1886..1916) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(2240..2828) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(2999..3859) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3860..3964) /gene="bla" /note="AmpR promoter" rep_origin 4296..4751 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 4892..4908 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 4915..2 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase"
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