载体基本信息 | |||
载体名称 | pAviTag C-His Kan | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 2359 bp | 载体类型 | Lucigen Vectors |
载体来源 | Lucigen | 拷贝数 | High copy number |
pAviTag C-His Kan 载体载体图谱
pAviTag C-His Kan 载体载体序列
LOCUS pAviTag_C-His_Ka 2359 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for Expresso(R) cloning to add C-terminal biotinylation and 6xHis tags, with rhamnose-inducible expression. ACCESSION . VERSION . KEYWORDS pAviTag C-His Kan. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2359) AUTHORS Lucigen TITLE Direct Submission REFERENCE 2 (bases 1 to 2359) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2359 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..119 /label=rhaB promoter /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" RBS 153..175 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 183..185 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 225..269 /label=AviTag(TM) /note="peptide tag that allows for enzymatic biotinylation" CDS 270..287 /label=6xHis /note="6xHis affinity tag" terminator 319..366 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 464..493 /label=T3Te terminator /note="phage T3 early transcription terminator" rep_origin 515..1102 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(1114..1141) /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(1168..1356) /label=rop /note="Rop protein, which maintains plasmids at low copy number" CDS complement(1360..2172) /label=KanR /note="aminoglycoside phosphotransferase" promoter complement(2173..2263) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator complement(2264..2295) /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator"
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