载体基本信息 | |||
载体名称 | pRham N-His Kan | 载体抗性 | Kanamycin |
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载体长度 | 2275 bp | 载体类型 | Lucigen Vectors |
载体来源 | Lucigen | 拷贝数 | High copy number |
pRham N-His Kan 载体载体图谱
pRham N-His Kan 载体载体序列
LOCUS pRham_N-His_Kan. 2275 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for Expresso(R) cloning to add an N-terminal 6xHis tag, with rhamnose-inducible expression. ACCESSION . VERSION . KEYWORDS pRham N-His Kan. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2275) AUTHORS Lucigen TITLE Direct Submission REFERENCE 2 (bases 1 to 2275) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2275 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..119 /label=rhaB promoter /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" RBS 153..175 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 183..185 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 186..203 /label=6xHis /note="6xHis affinity tag" terminator 235..282 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 380..409 /label=T3Te terminator /note="phage T3 early transcription terminator" rep_origin 431..1018 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(1030..1057) /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(1084..1272) /label=rop /note="Rop protein, which maintains plasmids at low copy number" CDS complement(1276..2088) /label=KanR /note="aminoglycoside phosphotransferase" promoter complement(2089..2179) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator complement(2180..2211) /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator"
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