载体基本信息 | |||
载体名称 | pSMART LCAmp | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2025 bp | 载体类型 | Lucigen Vectors |
载体来源 | Lucigen | 拷贝数 | High copy number |
pSMART LCAmp 载体载体图谱
pSMART LCAmp 载体载体序列
LOCUS Exported 2025 bp ds-DNA circular SYN 25-11-2013 DEFINITION Low-copy number vector with an ampicillin resistance marker for efficient blunt cloning of unstable sequences. ACCESSION AY090111 VERSION . KEYWORDS pSMART LCAmp SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2025) AUTHORS Lucigen TITLE Direct Submission COMMENT Linearize at HincII to make blunt cloning vector. FEATURES Location/Qualifiers source 1..2025 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 65..96 /note="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 97..199 /note="cat promoter" /note="promoter of the E. coli cat gene" CDS 200..1060 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" CDS 1061..1252 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" terminator 1276..1303 /note="T7Te terminator" /note="phage T7 early transcription terminator" rep_origin complement(1315..1902) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 1924..1953 /note="T3Te terminator" /note="phage T3 early transcription terminator"
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