- 载体名称:
- p3xFLAG-CMV-14
- 载体抗性:
- Ampicillin
- 载体长度:
- 6310 bp
- 载体类型:
- Mammalian Expression Vectors
- 复制子:
- ori
- 载体来源:
- Sigma-Aldrich
- 筛选标记:
- Neomycin/G418(Geneticin)
- 拷贝数:
- High copy number
- 启动子:
- CMV
- 克隆方法:
- Enzyme digestion and ligation
- 5'引物:
- M13 fwd
- 3'引物:
- M13 rev
p3xFLAG-CMV-14 载体载体图谱
p3xFLAG-CMV-14 载体载体序列
LOCUS p3xFLAG-CMV-14. 6310 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector with a neomycin resistance marker for expression of C-terminally 3xFLAG-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS p3xFLAG-CMV-14. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6310) AUTHORS Sigma-Aldrich TITLE Direct Submission REFERENCE 2 (bases 1 to 6310) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6310 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 141..157 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 318..697 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 924..990 /label=MCS /note="MCS" /note="multiple cloning site" CDS 993..1058 /label=3xFLAG /note="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" polyA_signal 1069..1691 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" promoter 1733..2030 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2084..2875 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 3530..3664 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(3670..3688) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3702..3718) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3726..3742) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3750..3780) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3795..3816) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4104..4692) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4866..5723) /label=AmpR /note="beta-lactamase" promoter complement(5724..5828) /label=AmpR promoter rep_origin 5855..6310 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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