pBI-CMV1, this is a mammalian bidirectional promoter vector for constitutively expressing two genes at similar levels.
- 载体名称:
- pBI-CMV1
- 载体抗性:
- Ampicillin
- 载体长度:
- 3114 bp
- 载体类型:
- Mammalian Expression Vectors
- 复制子:
- ori
- 载体来源:
- Clontech
- 拷贝数:
- High copy number
- 启动子:
- CMV
pBI-CMV1 载体载体图谱
pBI-CMV1 载体载体序列
LOCUS pBI-CMV1. 3114 bp DNA circular SYN 01-JAN-1980
DEFINITION Mammalian bidirectional promoter vector for constitutively
expressing two genes at similar levels.
ACCESSION .
VERSION .
KEYWORDS pBI-CMV1.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3114)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3114)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT The gene inserted into each MCS should contain start and stop
codons.
FEATURES Location/Qualifiers
source 1..3114
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 67..370
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 602..663
/label=MCS 1
/note="MCS 1"
/note="multiple cloning site 1"
polyA_signal 777..858
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1040..1628)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1802..2659)
/label=AmpR
/note="beta-lactamase"
promoter complement(2660..2764)
/label=AmpR promoter
polyA_signal complement(2797..2878)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 2986..3040
/label=MCS 2
/note="MCS 2"
/note="multiple cloning site 2"
promoter complement(3069..3106)
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
我们不提供该载体，载体信息仅供查询和参考。