pcDNA3.1+/C-HA 载体 (V011321#)

载体基本信息
载体名称 pcDNA3.1+/C-HA 载体抗性 Ampicillin
载体长度 5441 bp 载体类型 Mammalian Expression Vectors
载体来源 GenScript 筛选标记 Neomycin/G418(Geneticin)
拷贝数 High copy number

pcDNA3.1+/C-HA 载体载体图谱

pcDNA3.1+/C-HA5441 bp60012001800240030003600420048005400CMV enhancerCMV promoterT7 promoterMCSHAbGH poly(A) signalf1 oriSV40 promoterNeoR/KanRSV40 poly(A) signaloriAmpRAmpR promoter

pcDNA3.1+/C-HA 载体载体序列

LOCUS       Exported                5441 bp ds-DNA    circular SYN 21-5-2015
DEFINITION  Mammalian expression vector with a CMV promoter for expressing 
            C-terminally HA-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1+ C-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   GenScript
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..985
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             986..1012
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     polyA_signal    1038..1262
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1308..1736
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1750..2079
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1930..2065
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2146..2940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3114..3235
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3686..4274)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4445..5305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5306..5410)
                     /gene="bla"
                     /note="AmpR promoter"

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