载体基本信息 | |||
载体名称 | pcDNA3.1+/C-HA | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5441 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | GenScript | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pcDNA3.1+/C-HA 载体载体图谱
pcDNA3.1+/C-HA 载体载体序列
LOCUS Exported 5441 bp ds-DNA circular SYN 21-5-2015 DEFINITION Mammalian expression vector with a CMV promoter for expressing C-terminally HA-tagged proteins. ACCESSION . VERSION . KEYWORDS pcDNA3.1+ C-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5441) AUTHORS GenScript TITLE Direct Submission FEATURES Location/Qualifiers source 1..5441 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 895..985 /note="MCS" /note="multiple cloning site" CDS 986..1012 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" polyA_signal 1038..1262 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 1308..1736 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1750..2079 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 1930..2065 /note="SV40 ori" /note="SV40 origin of replication" CDS 2146..2940 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3114..3235 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3686..4274) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4445..5305) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5306..5410) /gene="bla" /note="AmpR promoter"
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