纽普生物

pET-17b载体

载体基本信息
载体名称 pET-17b 载体抗性 Ampicillin
载体长度 3306 bp 载体类型 pET & Duet Vectors (Novagen)
载体来源 Novagen (EMD Millipore) 拷贝数 High copy number

pET-17b载体质粒图谱

pET-17b载体质粒图谱

pET-17b载体载体序列

LOCUS       Exported                3306 bp ds-DNA    circular SYN 01-12-2013
DEFINITION  Bacterial vector for expression of N-terminally T7-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pET-17b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     terminator      28..75
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    141..228
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(237..269)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     RBS             278..283
                     /note="ribosome binding site"
     promoter        complement(331..349)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             858..1049
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2238..3098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3099..3203)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
       61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
      121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
      181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
      241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
      301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
      361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
      421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
      481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
      541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
      601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
      661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
      721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
      781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
      841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
      901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
      961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
     1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     3301 caagaa
//

pET-17b载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3306
常规描述Bacterial vector for expression of N-terminally T7-tagged proteins.
常规是否环形1
常规作者Novagen (EMD Millipore)
常规注释
来源实验宿主Escherichia coli1..3306
来源分子类型other DNA1..3306
来源种属synthetic DNA construct1..3306
终止子备注T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase28..75
其它特征区备注MCS,multiple cloning site141..228
编码区翻译起始位点1237..269
编码区备注T7 tag237..269
编码区产物epitope tag from a T7 major capsid protein237..269
编码区翻译序列MASMTGGQQMG237..269
核糖体结合位点备注ribosome binding site278..283
启动子备注T7 promoter,promoter for bacteriophage T7 RNA polymerase331..349
编码区翻译起始位点1858..1049
编码区基因rop858..1049
编码区备注rop858..1049
编码区产物Rop protein858..1049
编码区翻译序列MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL858..1049
复制起点方向LEFT1479..2067
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication1479..2067
编码区翻译起始位点12238..3098
编码区基因bla2238..3098
编码区备注AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics2238..3098
编码区产物beta-lactamase2238..3098
编码区翻译序列MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW2238..3098
启动子基因bla3099..3203
启动子备注AmpR promoter3099..3203

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