载体基本信息 | |||
载体名称 | pET-17b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3306 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-17b 载体载体图谱
pET-17b 载体载体序列
LOCUS Exported 3306 bp ds-DNA circular SYN 01-12-2013 DEFINITION Bacterial vector for expression of N-terminally T7-tagged proteins. ACCESSION . VERSION . KEYWORDS pET-17b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3306) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..3306 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 28..75 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 141..228 /note="MCS" /note="multiple cloning site" CDS complement(237..269) /codon_start=1 /product="epitope tag from a T7 major capsid protein" /note="T7 tag" /translation="MASMTGGQQMG" RBS 278..283 /note="ribosome binding site" promoter complement(331..349) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 858..1049 /codon_start=1 /gene="rop" /product="Rop protein" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin complement(1479..2067) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2238..3098) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3099..3203) /gene="bla" /note="AmpR promoter"
我们不提供该载体,载体信息仅供查询和参考。