- 载体名称:
- pET-19b
- 载体抗性:
- Ampicillin
- 载体长度:
- 5717 bp
- 载体类型:
- pET & Duet Vectors (Novagen)
- 复制子:
- ori
- 载体来源:
- Novagen (EMD Millipore)
- 拷贝数:
- High copy number
pET-19b 载体载体图谱
pET-19b 载体载体序列
LOCUS pET-19b. 5717 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for inducible expression of N-terminally 10xHis-tagged proteins with an enterokinase site. ACCESSION . VERSION . KEYWORDS pET-19b. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5717) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5717) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5717 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 10..38 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" terminator complement(213..260) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 319..335 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(336..350) /label=enterokinase site /note="enterokinase recognition and cleavage site" CDS complement(369..395) /label=9xHis /note="9xHis affinity tag" CDS complement(399..401) /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" RBS complement(408..430) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(445..469) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(470..488) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 797..874 /label=lacI promoter CDS 875..1954 /label=lacI /note="lac repressor" protein_bind 1970..1991 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3269..3457 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3562..3704 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3890..4478) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4652..5509) /label=AmpR /note="beta-lactamase" promoter complement(5510..5614) /label=AmpR promoter
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