纽普生物

pET-23a(+)载体

载体基本信息
载体名称 pET-23a(+) 载体抗性 Ampicillin
载体长度 3666 bp 载体类型 pET & Duet Vectors (Novagen)
载体来源 Novagen (EMD Millipore) 拷贝数 High copy number

pET-23a(+)载体质粒图谱

pET-23a(+)载体质粒图谱

pET-23a(+)载体载体序列

LOCUS       Exported                3666 bp ds-DNA    circular SYN 02-12-2013
DEFINITION  Bacterial vector for expression of N-terminally T7-tagged proteins. 
            For other reading frames, use pET-23b(+) or pET-23c(+).
ACCESSION   .
VERSION     .
KEYWORDS    pET-23a(+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     terminator      26..73
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    158..203
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(207..239)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     RBS             248..253
                     /note="ribosome binding site"
     promoter        complement(301..319)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             828..1019
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(1449..2037)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2208..3068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3069..3173)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(3200..3655)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
      241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
      361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
      481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
      541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
      601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
      661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
      721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
      781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
      841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
      901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
      961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     3661 tcgcca
//

pET-23a(+)载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度3666
常规描述Bacterial vector for expression of N-terminally T7-tagged proteins. For other reading frames, use pET-23b(+) or pET-23c(+).
常规是否环形1
常规作者Novagen (EMD Millipore)
常规注释
来源实验宿主Escherichia coli1..3666
来源分子类型other DNA1..3666
来源种属synthetic DNA construct1..3666
终止子备注T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase26..73
编码区翻译起始位点1140..157
编码区备注6xHis140..157
编码区产物6xHis affinity tag140..157
编码区翻译序列HHHHHH140..157
其它特征区备注MCS,multiple cloning site158..203
编码区翻译起始位点1207..239
编码区备注T7 tag207..239
编码区产物epitope tag from a T7 major capsid protein207..239
编码区翻译序列MASMTGGQQMG207..239
核糖体结合位点备注ribosome binding site248..253
启动子备注T7 promoter,promoter for bacteriophage T7 RNA polymerase301..319
编码区翻译起始位点1828..1019
编码区基因rop828..1019
编码区备注rop828..1019
编码区产物Rop protein828..1019
编码区翻译序列MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL828..1019
复制起点方向LEFT1449..2037
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication1449..2037
编码区翻译起始位点12208..3068
编码区基因bla2208..3068
编码区备注AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics2208..3068
编码区产物beta-lactamase2208..3068
编码区翻译序列MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW2208..3068
启动子基因bla3069..3173
启动子备注AmpR promoter3069..3173
复制起点方向LEFT3200..3655
复制起点备注f1 ori,f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis3200..3655

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