载体基本信息 | |||
载体名称 | pET-24a(+) | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 5310 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-24a(+) 载体载体图谱
pET-24a(+) 载体载体序列
LOCUS pET-24a(+). 5310 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with a kanamycin resistance marker for inducible expression of T7-tagged proteins. For other reading frames, use pET-24b(+) or pET-24c(+). ACCESSION . VERSION . KEYWORDS pET-24a(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5310) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5310) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5310 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" misc_feature 158..203 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(207..239) /label=T7 tag (gene 10 leader) /note="leader peptide from bacteriophage T7 gene 10" RBS complement(247..269) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(284..308) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(309..327) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 636..713 /label=lacI promoter CDS 714..1793 /label=lacI /note="lac repressor" protein_bind 1809..1830 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 2605..2793 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 2898..3040 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3226..3814) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 3936..4748 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(4844..5299) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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