pET-28c(+)载体质粒图谱
/pET-28c(+) Map.png)
pET-28c(+)载体载体序列
LOCUS Exported 5367 bp ds-DNA circular SYN 02-12-2013
DEFINITION Bacterial vector for expression of N-terminally 6xHis-tagged
proteins with a thrombin site. For other reading frames, use
pET-28a(+) or pET-28b(+).
ACCESSION .
VERSION .
KEYWORDS pET-28c(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5367)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
COMMENT
FEATURES Location/Qualifiers
source 1..5367
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 26..73
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 158..203
/note="MCS"
/note="multiple cloning site"
CDS complement(205..237)
/codon_start=1
/product="epitope tag from a T7 major capsid protein"
/note="T7 tag"
/translation="MASMTGGQQMG"
CDS complement(241..258)
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
CDS complement(268..285)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS complement(295..297)
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
RBS 305..310
/note="ribosome binding site"
protein_bind 341..365
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(366..384)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 693..770
/gene="lacI"
/note="lacI promoter"
/note="
"
CDS 771..1853
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
CDS 2662..2853
/codon_start=1
/gene="rop"
/product="Rop protein"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
rep_origin complement(3283..3871)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 3993..4808
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(4901..5356)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
361 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg
421 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
481 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
541 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
601 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
661 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
721 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
781 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
841 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
901 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
961 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
1021 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
1081 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
1141 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
1201 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
1261 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
1321 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
1381 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
1441 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
1501 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
1561 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
1621 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
1681 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
1741 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
1801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
1861 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
1921 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
1981 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
2041 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
2101 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
2161 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg
2221 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
2281 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
2341 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
2401 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
2461 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
2521 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
2581 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
2641 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
2701 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
2761 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
2821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
2881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
2941 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
3001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat
3061 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
3121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3721 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt
3961 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt
4021 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
4081 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
4141 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
4201 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
4261 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
4321 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
4381 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
4441 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
4501 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
4561 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
4621 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
4681 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
4741 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
4801 ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa
4861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt
4921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
4981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
5041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
5101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
5161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
5221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
5281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
5341 ccgctacagg gcgcgtccca ttcgcca
//
pET-28c(+)载体载体特征
一级属性 |
二级属性 |
属性值 |
起始位置 |
常规 | 长度 | 5367 | |
常规 | 描述 | Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a(+) or pET-28b(+). | |
常规 | 是否环形 | 1 | |
常规 | 作者 | Novagen (EMD Millipore) | |
常规 | 注释 | | |
来源 | 实验宿主 | Escherichia coli | 1..5367 |
来源 | 分子类型 | other DNA | 1..5367 |
来源 | 种属 | synthetic DNA construct | 1..5367 |
终止子 | 备注 | T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase | 26..73 |
编码区 | 翻译起始位点 | 1 | 140..157 |
编码区 | 备注 | 6xHis | 140..157 |
编码区 | 产物 | 6xHis affinity tag | 140..157 |
编码区 | 翻译序列 | HHHHHH | 140..157 |
其它特征区 | 备注 | MCS,multiple cloning site | 158..203 |
编码区 | 翻译起始位点 | 1 | 205..237 |
编码区 | 备注 | T7 tag | 205..237 |
编码区 | 产物 | epitope tag from a T7 major capsid protein | 205..237 |
编码区 | 翻译序列 | MASMTGGQQMG | 205..237 |
编码区 | 翻译起始位点 | 1 | 241..258 |
编码区 | 备注 | thrombin site | 241..258 |
编码区 | 产物 | thrombin recognition and cleavage site | 241..258 |
编码区 | 翻译序列 | LVPRGS | 241..258 |
编码区 | 翻译起始位点 | 1 | 268..285 |
编码区 | 备注 | 6xHis | 268..285 |
编码区 | 产物 | 6xHis affinity tag | 268..285 |
编码区 | 翻译序列 | HHHHHH | 268..285 |
编码区 | 翻译起始位点 | 1 | 295..297 |
编码区 | 备注 | ATG | 295..297 |
编码区 | 产物 | start codon | 295..297 |
编码区 | 翻译序列 | M | 295..297 |
核糖体结合位点 | 备注 | ribosome binding site | 305..310 |
蛋白结合位点 | 嵌合范围 | lac repressor encoded by lacI | 341..365 |
蛋白结合位点 | 备注 | lac operator,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). | 341..365 |
启动子 | 备注 | T7 promoter,promoter for bacteriophage T7 RNA polymerase | 366..384 |
启动子 | 基因 | lacI | 693..770 |
启动子 | 备注 | lacI promoter, | 693..770 |
编码区 | 翻译起始位点 | 1 | 771..1853 |
编码区 | 基因 | lacI | 771..1853 |
编码区 | 备注 | lacI,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). | 771..1853 |
编码区 | 产物 | lac repressor | 771..1853 |
编码区 | 翻译序列 | MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ | 771..1853 |
编码区 | 翻译起始位点 | 1 | 2662..2853 |
编码区 | 基因 | rop | 2662..2853 |
编码区 | 备注 | rop | 2662..2853 |
编码区 | 产物 | Rop protein | 2662..2853 |
编码区 | 翻译序列 | MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL | 2662..2853 |
复制起点 | 方向 | LEFT | 3283..3871 |
复制起点 | 备注 | ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication | 3283..3871 |
编码区 | 翻译起始位点 | 1 | 3993..4808 |
编码区 | 基因 | aph(3')-Ia | 3993..4808 |
编码区 | 备注 | KanR,confers resistance to kanamycin | 3993..4808 |
编码区 | 产物 | aminoglycoside phosphotransferase | 3993..4808 |
编码区 | 翻译序列 | MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF | 3993..4808 |
复制起点 | 方向 | LEFT | 4901..5356 |
复制起点 | 备注 | f1 ori,f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis | 4901..5356 |
我们不提供该载体,载体信息仅供查询和参考。