| 载体基本信息 | |||
| 载体名称 | pET-32a(+) | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 5900 bp | 载体类型 | pET & Duet Vectors (Novagen) |
| 载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-32a(+) 载体载体图谱
pET-32a(+) 载体载体序列
LOCUS pET-32a(+). 5900 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector for expressing thioredoxin fusion proteins with an
enterokinase site. For other reading frames, use pET-32b(+) or
pET-32c(+).
ACCESSION .
VERSION .
KEYWORDS pET-32a(+).
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5900)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5900)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5900
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label=6xHis
/note="6xHis affinity tag"
misc_feature 158..217
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS complement(219..233)
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
CDS complement(249..293)
/label=S-Tag
/note="affinity and epitope tag derived from pancreatic
ribonuclease A"
CDS complement(300..317)
/label=thrombin site
/note="thrombin recognition and cleavage site"
CDS complement(327..344)
/label=6xHis
/note="6xHis affinity tag"
CDS complement(366..692)
/label=TrxA
/note="E. coli thioredoxin"
RBS complement(700..722)
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind complement(737..761)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(762..780)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 1093..1170
/label=lacI promoter
CDS 1171..2250
/label=lacI
/note="lac repressor"
protein_bind 2266..2287
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 3062..3250
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 3355..3497
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3683..4271)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4445..5302)
/label=AmpR
/note="beta-lactamase"
promoter complement(5303..5407)
/label=AmpR promoter
rep_origin complement(5434..5889)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。