载体基本信息 | |||
载体名称 | pET-3b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4639 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-3b 载体载体图谱
pET-3b 载体载体序列
LOCUS Exported 4639 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Bacterial expression vector pET-3b, complete sequence. ACCESSION LT727575 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4639) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 4639) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 4639) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4639 /organism="Bacterial expression vector pET-3b" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 1458." /db_xref="taxon:1944667" promoter 10..38 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" regulatory complement(404..451) /regulatory_class="terminator" /note="T7T" terminator 404..451 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(518..550) /codon_start=1 /note="unnamed protein product; T710f" /protein_id="SJL88896.1" /translation="MASMTGGQQMG" misc_feature complement(551..613) /label=5UTR /note="5UTR" RBS 558..580 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" promoter complement(612..630) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory complement(614..630) /regulatory_class="promoter" /note="T7" CDS 2191..2382 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature 2484..2626 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(2628..3030) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(2812..3400) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3571..4431) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL88897.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4432..4536) /gene="bla" /label=AmpR promoter
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