纽普生物

pET-3c载体

载体基本信息
载体名称 pET-3c 载体抗性 Ampicillin
载体长度 4638 bp 载体类型 pET & Duet Vectors (Novagen)
载体来源 Novagen (EMD Millipore) 拷贝数 High copy number

pET-3c载体质粒图谱

pET-3c载体质粒图谱

pET-3c载体载体序列

LOCUS       Exported                4638 bp ds-DNA    circular SYN 01-12-2013
DEFINITION  Bacterial expression vector with a BamHI cloning site. For 
            alternative reading frames, use pET-3a or pET-3b.
ACCESSION   .
VERSION     .
KEYWORDS    pET-3c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
  JOURNAL   Exported 2017/2/28 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     terminator      404..451
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(517..549)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     RBS             558..563
                     /note="ribosome binding site"
     promoter        complement(611..629)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2190..2381
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(2811..3399)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3570..4430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4431..4535)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
      541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
      601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
      961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4561 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4621 ccctttcgtc ttcaagaa
//

pET-3c载体载体特征

一级属性 二级属性 属性值 起始位置
常规长度4638
常规描述Bacterial expression vector with a BamHI cloning site. For alternative reading frames, use pET-3a or pET-3b.
常规是否环形1
常规作者Novagen (EMD Millipore)
常规注释
来源实验宿主Escherichia coli1..4638
来源分子类型other DNA1..4638
来源种属synthetic DNA construct1..4638
终止子备注T7 terminator,transcription terminator for bacteriophage T7 RNA polymerase404..451
编码区翻译起始位点1517..549
编码区备注T7 tag517..549
编码区产物epitope tag from a T7 major capsid protein517..549
编码区翻译序列MASMTGGQQMG517..549
核糖体结合位点备注ribosome binding site558..563
启动子备注T7 promoter,promoter for bacteriophage T7 RNA polymerase611..629
编码区翻译起始位点12190..2381
编码区基因rop2190..2381
编码区备注rop2190..2381
编码区产物Rop protein2190..2381
编码区翻译序列MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL2190..2381
复制起点方向LEFT2811..3399
复制起点备注ori,high-copy-number ColE1/pMB1/pBR322/pUC origin of replication2811..3399
编码区翻译起始位点13570..4430
编码区基因bla3570..4430
编码区备注AmpR,confers resistance to ampicillin, carbenicillin, and related antibiotics3570..4430
编码区产物beta-lactamase3570..4430
编码区翻译序列MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW3570..4430
启动子基因bla4431..4535
启动子备注AmpR promoter4431..4535

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