载体基本信息 | |||
载体名称 | pET-42a(+) | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 5930 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-42a(+) 载体载体图谱
pET-42a(+) 载体载体序列
LOCUS pET-42a(+). 5930 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins with a Factor Xa site. For other reading frames, use pET-42b(+) or pET-42c(+). ACCESSION . VERSION . KEYWORDS pET-42a(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5930) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5930) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5930 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(150..173) /label=8xHis /note="8xHis affinity tag" misc_feature 174..264 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(265..276) /label=Factor Xa site /note="Factor Xa recognition and cleavage site" CDS complement(307..351) /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS complement(367..384) /label=thrombin site /note="thrombin recognition and cleavage site" CDS complement(394..411) /label=6xHis /note="6xHis affinity tag" CDS complement(439..1092) /label=GST /note="glutathione S-transferase from Schistosoma japonicum" RBS complement(1100..1122) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1137..1161) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1162..1180) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1493..1570 /label=lacI promoter CDS 1571..2650 /label=lacI /note="lac repressor" protein_bind 2666..2687 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3225..3413 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3518..3660 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3846..4434) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 4556..5368 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(5464..5919) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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