The pRSFDuet-1 is a bacterial vector with an RSF 1030 origin for the co-expression of two genes.
载体基本信息 | |||
载体名称 | pRSFDuet-1 | 载体抗性 | Kanamycin |
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载体长度 | 3829 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pRSFDuet-1 载体载体图谱
pRSFDuet-1 载体载体序列
LOCUS Exported 3829 bp ds-DNA circular SYN 26-12-2012 DEFINITION Bacterial vector with an RSF 1030 origin for the co-expression of two genes. ACCESSION . VERSION . KEYWORDS pRSFDuet-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3829) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission FEATURES Location/Qualifiers source 1..3829 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" protein_bind 3..27 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 58..63 /note="ribosome binding site" misc_feature 69..168 /note="MCS-1" /note="multiple cloning site 1" CDS 71..73 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 83..100 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" promoter 214..232 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 233..257 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 286..291 /note="ribosome binding site" misc_feature 297..438 /note="MCS-2" /note="multiple cloning site 2" CDS 300..302 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 366..410 /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /note="S-Tag" /translation="KETAAAKFERQHMDS" terminator 462..509 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(739..1554) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(1555..1646) /gene="bla" /note="AmpR promoter" rep_origin complement(1662..2411) /direction=LEFT /note="RSF ori" /note="Plasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." CDS complement(2607..3689) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(3690..3767) /gene="lacI" /note="lacI promoter" /note=" " promoter 3813..2 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase"
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