载体基本信息 | |||
载体名称 | pCAMBIA3200 | 载体抗性 | Chloramphenicol |
---|---|---|---|
载体长度 | 8581 bp | 载体类型 | Plant Vectors |
载体来源 | Cambia | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pCAMBIA3200 载体载体图谱
pCAMBIA3200 载体载体序列
LOCUS Exported 8581 bp ds-DNA circular SYN 11-1-2016 DEFINITION Agrobacterium binary vector for plant transformation, with bialophos/phosphinothricin resistance and chloramphenicol resistance genes. ACCESSION . VERSION . KEYWORDS pCAMBIA3200 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8581) AUTHORS Cambia TITLE Direct Submission FEATURES Location/Qualifiers source 1..8581 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 1219..1848 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /note="pVS1 StaA" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2277..3350 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA" /note=" " /protein_id=" " /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3416..3610 /note="pVS1 oriV" /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 3954..4094 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(4280..4868) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5139..5798) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" misc_feature 6326..6350 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" polyA_signal 6428..6602 /note="CaMV poly(A) signal" /note="cauliflower mosaic virus polyadenylation signal" CDS complement(6609..7160) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /function=" " /note="BlpR" /note="confers resistance to bialophos or phosphinothricin" /protein_id=" " /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(7205..7882) /note="CaMV 35S promoter (enhanced)" /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 8109..8139 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 8147..8163 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8171..8187 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 8183..8416 /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 8197..8253 /gene=" " /note="MCS" /note="pUC18/19 multiple cloning site" primer_bind complement(8257..8273) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 8476..8500 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA"
我们不提供该载体,载体信息仅供查询和参考。